A human SARS-CoV neutralizing antibody against epitope on S2 protein.
Identifieur interne : 004951 ( Main/Exploration ); précédent : 004950; suivant : 004952A human SARS-CoV neutralizing antibody against epitope on S2 protein.
Auteurs : Jinzhu Duan [République populaire de Chine] ; Xiyun Yan ; Xueming Guo ; Wuchun Cao ; Wei Han ; Cai Qi ; Jing Feng ; Dongling Yang ; Guangxia Gao ; Gang JinSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Antibodies, Epitopes, B-Lymphocyte, Viral Envelope Proteins.
- immunology : B-Lymphocytes, SARS Virus.
- methods : Epitope Mapping, Immunoassay, Neutralization Tests.
- Humans, Peptide Library.
Abstract
An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, K(d) = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023-1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.
DOI: 10.1016/j.bbrc.2005.05.089
PubMed: 15939399
Affiliations:
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Le document en format XML
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sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Microbial Resources, Institute of Microbiology, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Microbial Resources, Institute of Microbiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name></author><author><name sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name></author><author><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></author><author><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name></author><author><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies (immunology)</term><term>B-Lymphocytes (immunology)</term><term>Epitope Mapping (methods)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Humans</term><term>Immunoassay (methods)</term><term>Neutralization Tests (methods)</term><term>Peptide Library</term><term>SARS Virus (immunology)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps (immunologie)</term><term>Banque de peptides</term><term>Cartographie épitopique ()</term><term>Dosage immunologique ()</term><term>Déterminants antigéniques des lymphocytes B (immunologie)</term><term>Humains</term><term>Lymphocytes B (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Tests de neutralisation ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Epitopes, B-Lymphocyte</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Déterminants antigéniques des lymphocytes B</term><term>Lymphocytes B</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term><term>Immunoassay</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Cartographie épitopique</term><term>Dosage immunologique</term><term>Humains</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, K(d) = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023-1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name><name sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Duan, Jinzhu" sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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